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SHORT COMMUNICATION
Year : 2015  |  Volume : 4  |  Issue : 4  |  Page : 267-270

Cytotoxicity activities of chloroform extract of Cichorium intybus seed against HCT-15 and Vero cell line


Department of Pharmacology, NGSPMS, College of Pharmacy, Brahma Valley Educational Campus, Nashik, Maharashtra, India

Correspondence Address:
Prashant Y Mali
Department of Pharmacology, NGSPMS, College of Pharmacy, Anjaneri, Trimbak Road, Nashik - 422 213, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2278-344X.167647

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Background: Cichorium intybus L., (Asteraceae) is well-known as a coffee substitute but is also widely used medicinally to treat various ailments ranging from wounds to diabetes. Other plant parts are also used for liver and cancer disorder. Objective: The objective was to study the cytotoxic potential of chloroform extract of C. intybus seed against HCT-15 and Vero (normal) cell line. Materials and Methods: Fourier transform infrared spectroscopy (FTIR) analysis of the extract was performed. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay was used for evaluation of the cytotoxic potential of chloroform extract of C. intybus seed. Doxorubicin was considered as standard reference drug. The concentrations 1000–0.05 μg/ml was used in the experiment. Result and Discussion: FTIR spectrum showed 1025.363, 1083.126, 1291.366, 1389.144, and 1569.294 peaks/centers in the wavelength region of 4,000.00–650.00 cm−1. The chloroform extract of C. intybus seed and doxorubicin was showed 1411.37 μg/ml and 460.13 μg/ml 50% cell growth inhibition (IC50) against the HCT-15 cell line. Both extract and doxorubicin were safe against the Vero (normal) cell line. Conclusion: It can be concluded that the chloroform extract of C. intybus seed was not efficient against the HCT-15 cell line at the concentrations used in the experiment. Furthermore, there is no need to explore the said studies by in vivo models.


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