Home Print this page Email this page
Users Online: 1387
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 


 
 Table of Contents  
LETTERS TO EDITOR
Year : 2015  |  Volume : 4  |  Issue : 4  |  Page : 272-273

Study of biofilm production and antimicrobial sensitivity pattern of urinary tract infection causing Acinetobacter baumannii and Pseudomonas aeruginosa in a tertiary care hospital


1 Department of Microbiology, Pandit B.D. Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
2 Department of Neuroanaesthesiology, All Institute of Medical Sciences, New Delhi, India

Date of Web Publication20-Oct-2015

Correspondence Address:
Pragyan Swagatika Panda
Department of Microbiology, Pandit B.D. Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2278-344X.167655

Rights and Permissions

How to cite this article:
Panda PS, Chaudhary U, Dube SK. Study of biofilm production and antimicrobial sensitivity pattern of urinary tract infection causing Acinetobacter baumannii and Pseudomonas aeruginosa in a tertiary care hospital. Int J Health Allied Sci 2015;4:272-3

How to cite this URL:
Panda PS, Chaudhary U, Dube SK. Study of biofilm production and antimicrobial sensitivity pattern of urinary tract infection causing Acinetobacter baumannii and Pseudomonas aeruginosa in a tertiary care hospital. Int J Health Allied Sci [serial online] 2015 [cited 2019 Sep 20];4:272-3. Available from: http://www.ijhas.in/text.asp?2015/4/4/272/167655

Sir,

Urinary tract infection (UTI) now-a-days is one of the most common nosocomial infections. Both Pseudomonasaeruginosa (PA) and Acinetobacterbaumannii (AB) can cause UTI and they have high potential to form biofilm that is responsible for their high survival potential, antibiotic resistance, and virulence.[1] Contrary to previous belief, AB is an important emerging nosocomial pathogen worldwide and its infections have high mortality rates at present.[2] But, studies on the biofilm production by UTI causing PA and AB are scarce. So we prospectively investigated biofilm production and antimicrobial susceptibility pattern of UTI causing PA and AB.

Total 50 isolates of PA and 10 isolates of AB from the nosocomial urinary samples were investigated for biofilm production by tissue culture plate assay.[3] The antimicrobial susceptibility of the biofilm producing isolates was done by Kirby-Bauer disc diffusion technique. Biofilm production was detected in 58% and 70% isolates of PA and AB, respectively. All the biofilm producing PA and AB isolates were multidrug resistant (MDR). Both PA and AB isolates were highly resistant to ceftazidime, cefepime, gentamicin, and ciprofloxacin. However, in contrast to PA (highest sensitivity to imipenem), AB showed maximum sensitivity to piperacillin-tazobactam and netilimicin [Table 1].
Table 1: Antibitotic resistance pattern of the biofilm producing Pseudomonas spp. (n = 29) Acinetobacter spp. (n = 7) as detected by TCP method

Click here to view


Biofilm production by PA and AB isolates were reported to be 75%[4] and 66.7%[5], respectively, in previous studies. All (100%) the PA isolates in the study by Nagaveni et al.[4] were resistant to cefepime, ceftazidime, and ciprofloxacin, which is higher than that in our study. The resistance to cefepime, ceftazidime by AB isolates in the study by Rao et al.,[5] were 30.9% and 36.3%, respectively, which is lower than that in our study. However, ciprofloxacin resistance in their study was similar to our study (72.7%).[5] Moreover, the resistance of biofilm producing PA to imipenem has been reported to be 20%,[4] which is similar to our study, result (27.5%). The resistance of biofilm producing AB to netilimicin has been reported to be 27.5%,[5] which different from our study result (14.2%).

Gurung et al.,[1] reported a study similar to ours. However, the method used for biofilm detection in their study was less accurate (i.e. tube method) leading to detection of biofilm in only 25% of AB and 40% of PA isolates in contrast to our results. Secondly, they have studied biofilm production in various clinical isolates and the urinary sample in their study was only 10 in contrast to 60 isolates in our study. Lastly, among the biofilm producers 57% of PA and 73% of AB were MDR, but in our study all the biofilm producing isolates (100%) were MDR.

Thus, we conclude that biofilm production is very common among UTI causing PA and AB now-a-days and the biofilm producing PA and AB are MDR. The UTI causing MDR PA is sensitive to imipenem whereas, AB is sensitive to netilimicin and piperacillin-tazobactam.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
  References Top

1.
Gurung J, Khyriem AB, Banik A, Lyngdoh WV, Choudhury B, Bhattacharyya P. Association of biofilm production with multidrug resistance among clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from intensive care unit. Indian J Crit Care Med 2013;17:214-8.  Back to cited text no. 1
[PUBMED]  Medknow Journal  
2.
Doughari HJ, Ndakidemi PA, Human IS, Benade S. The ecology, biology and pathogenesis of Acinetobacter spp.: An overview. Microbes Environ 2011;26:101-12.  Back to cited text no. 2
    
3.
Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of Staphylococci: An evaluation of three different screening methods. Indian J Med Microbiol 2006;24:25-9.  Back to cited text no. 3
[PUBMED]  Medknow Journal  
4.
Nagaveni S, Rajeshwari H, Oli AK, Patil SA, Chandrakanth RK. Evaluation of biofilm forming ability of the multidrug resistant Pseudomonas aeruginosa. Bioscan 2010;5:563-6.  Back to cited text no. 4
    
5.
Rao RS, Karthika RU, Singh SP, Shashikala P, Kanungo R, Jayachandran S, et al. Correlation between biofilm production and multiple drug resistance in imipenem resistant clinical isolates of Acinetobacter baumannii. Indian J Med Microbiol 2008;26:333-7.  Back to cited text no. 5
[PUBMED]  Medknow Journal  



 
 
    Tables

  [Table 1]


This article has been cited by
1 Proteomic analysis reveals modulation of iron homeostasis and oxidative stress response in Pseudomonas aeruginosa PAO1 by curcumin inhibiting quorum sensing regulated virulence factors and biofilm production
Sivasamy Sethupathy,Krishnan Ganesh Prasath,Sivagnanam Ananthi,Sundarasamy Mahalingam,Samiraj Yesu Balan,Shunmugiah Karutha Pandian
Journal of Proteomics. 2016;
[Pubmed] | [DOI]



 

Top
 
 
  Search
 
Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

 
  In this article
References
Article Tables

 Article Access Statistics
    Viewed551    
    Printed1    
    Emailed0    
    PDF Downloaded131    
    Comments [Add]    
    Cited by others 1    

Recommend this journal