Home Print this page Email this page
Users Online: 173
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2018  |  Volume : 7  |  Issue : 2  |  Page : 98-103

Fetal hemoglobin gene expression in patients with sickle cell disease in North Central Nigeria


1 Department of Medical Laboratory Services, University of Abuja Teaching Hospital, Abuja, Nigeria
2 Department of Medical Microbiology and Parasitology, College of Health Sciences, University of Ilorin, Ilorin, Nigeria
3 Department of Medical Laboratory Science, University of Maiduguri, Maiduguri, Nigeria
4 Department of Medical Laboratory Science (Haematology Unit), Ambrose Alli University, Edo State, Nigeria
5 Department of Haematology, College of Veterinary and Medical Laboratory Science, Vom, Plateau State, Nigeria
6 Department of Family Medicine, University of Abuja Teaching Hospital, Abuja, Nigeria

Correspondence Address:
Mr. Idris Abdullahi Nasir
Department of Medical Laboratory Services, University of Abuja Teaching Hospital, PMB 228 Gwagwalada, FCT Abuja
Nigeria
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijhas.IJHAS_135_1

Rights and Permissions

BACKGROUND: Fetal hemoglobin (HbF) plays a dominant role in ameliorating morbidity and mortality associated with sickle cell disease (SCD). OBJECTIVE: This study evaluated the distribution pattern of HbF and total Hb concentration among 75 participants with homozygous sickle cell trait (HbSHbS) as test cases and 71 with homozygous normal trait (HbAHbA) as controls. MATERIALS AND METHODS: Ethylenediaminetetraacetic acid-anticoagulated blood samples were collected from all participants. They were tested for HbF and HbA fractions using high-performance liquid chromatography, while total Hb concentration was determined by cynomethemoglobin technique. RESULTS: Participants with HbSHbS genotype showed mean ± standard error of mean (SEM) of HbF levels of 6.5 ± 0.8%, HbA1 showed mean ± SEM of 2.6 ± 0.3%, and HbA2 showed mean ± SEM of 4.9 ± 0.1%. Those with HbAHbA (control participants) genotype showed mean ± SEM of HbF levels of 0.5 ± 0.04%, HbA1 showed mean ± SEM of 87.3 ± 0.4%, and HbA2 showed mean ± SEM of 3.2 ± 0.1%, while the mean ± SEM Hb concentration of test cases was 6.5 ± 0.16 g/dl and that of controls was 12.32 ± 0.13 g/dl. The total Hb concentration of sickle cell patients was significantly lower than that of nonsickle cell patients. There was a positive correlation of Hb concentration (g/dl) with HbSHbS gene expression. CONCLUSION: Findings from this study revealed that the lesser episodes of sickle cell crisis, the lower the HbF expression and higher the total Hb concentration. Hence, it is recommended that the determination of HbF, HbA1, and HbA2 levels be considered in conjunction with other routine complete blood count and hematology tests in the diagnosis and clinical management of SCD.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed788    
    Printed3    
    Emailed0    
    PDF Downloaded93    
    Comments [Add]    

Recommend this journal