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ORIGINAL ARTICLE
Year : 2019  |  Volume : 8  |  Issue : 3  |  Page : 187-192

Molecular characterization of rotavirus genotype-A in children with acute diarrhea attending a tertiary hospital in Ilorin, Nigeria


1 Department of Medical Microbiology and Parasitology, University of Ilorin Teaching Hospital, Ilorin, Nigeria
2 Department of Medical Laboratory Science, College of Medical Sciences, Ahmadu Bello University, Zaria, Kaduna, Nigeria
3 Department of Medical Laboratory Science, University of Calabar, Calabar, Nigeria
4 Department of Medicine, Ahmadu Bello University, Zaria, Kaduna, Nigeria
5 Department of Medicine, Faculty of Health Sciences, National University of Samoa, Samoa
6 Department of Medical Microbiology and Parasitology, University of Abuja, Abuja, Nigeria
7 Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, Nigeria
8 Department of Medical Microbiology, Federal School of Medical Laboratory Technology, Jos, Nigeria

Correspondence Address:
Dr. Idris Nasir Abdullahi
Department of Medical Laboratory Science, College of Medical Sciences, Ahmadu Bello University, PMB 06 Shika, Zaria
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijhas.IJHAS_94_18

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BACKGROUND: Despite indications that severe rotavirus diarrhea in children under <5 years of age is a major public health problem, only limited specific data on rotavirus burden are available in Sub-Saharan Africa. This study aimed to carry out molecular characterization of circulating rotavirus strains causing acute diarrhea among under-five children attending University of Ilorin Teaching Hospital (UITH), Ilorin, Nigeria. MATERIALS AND METHODS: Ninety-three under-five children who are hospitalized with severe diarrhea were enrolled and their stool samples were collected for the detection and subsequent characterization for G and P rotavirus serotypes. RESULTS: Of a total of 93 samples comprising 54 (58.1%) males and 39 (41.9%) females, 25 (26.9%) samples were positive for rotavirus by ELISA. Genotyping by RT-PCR was done on 25 samples. The most prevalent type-able VP7 G types were G9 (28%), G1 (24%), followed by G12 (20%), G2 (12%), and G10 (4%); on the other hand, the most prevalent type-able VP4 P types are P8 (48%), P4 (24%), and P6 (16%). The most common G-P combination was G9P (4) (20%) followed by G12P (8) and G1P (4) (16%, respectively); then GNTP (8) and G1P (6) (8%, respectively); and G9P (8), G12P (4), G10P (6), G2P (8), and G2P (6) (4%, respectively). The rotaviruses isolated from these children were from those <24 months of age (100%). There is statistical relationship between the prevalence of rotavirus infection with age (P = 0.033) but not with gender (P = 0.765). CONCLUSION: This study highlights the rotavirus disease burden and diversity of rotavirus strains circulating in UITH. Continued sentinel surveillance will provide useful information to policy-makers with regard to rotavirus vaccine introduction.


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