TY - JOUR A1 - Tayal, Ruchi A1 - Baveja, Sujata A1 - De, Anuradha T1 - Analysis of biofilm formation and antibiotic susceptibility pattern of uropathogens in patients admitted in a tertiary care hospital in India Y1 - 2015/10/1 JF - International Journal of Health & Allied Sciences JO - Int J Health Allied Sci SP - 247 EP - 252 VL - 4 IS - 4 UR - https://www.ijhas.in/article.asp?issn=2278-344X;year=2015;volume=4;issue=4;spage=247;epage=252;aulast=Tayal DO - 10.4103/2278-344X.167648 N2 - Background: Microorganisms attach to surfaces and produce polysaccharides resulting in the formation of biofilms and providing an ideal niche for the exchange of genetic material leading to the emergence of drug-resistant pathogens. Biofilms can develop on anatomical surfaces and implants producing chronic and intractable infections. Aims: Detection of biofilm formation and comparison of antibiotic resistance between biofilm producers and nonproducers. Study Design: Prospective study in which urine specimens from adult patients with urinary tract infection (UTI) during the period of the study were analyzed (1 year). Materials and Methods: Mid-stream clean catch urine from noncatheterized and urine aspirated from in-dwelling urinary catheter in catheterized patients were taken for microbiological processing. Wet mounts, Gram-staining, and urine culture were done. Biofilm formation was detected by tissue culture plate method (TCPM). Statistical Analysis: Chi-square test and mid "P" test were used to analyze the data. A value ofP<0.05 was taken as significant. Results: Gram-negative organisms predominated (89%). Biofilm production was detected in 27% isolates. Maximum biofilm production was seen in Enterococcus spp. (71%), followed by Escherichia coli (26%). Biofilm-producing isolates demonstrated higher antibiotic resistance. All the biofilm-producing Enterococcus spp. showed high-level aminoglycoside resistance. The biofilm-producing isolates of Pseudomonas aeruginosa and Klebsiella pneumoniae demonstrated multi-drug resistance. Conclusions: TCPM is an economical phenotypic method which can be used routinely to detect biofilm formation. Biofilms contribute to an increased resistance to antibiotics used for the treatment of UTIs. Therefore, detection of biofilms is recommended for all patients presenting with chronic or recurrent disease. ER -