International Journal of Health & Allied Sciences

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 3  |  Issue : 1  |  Page : 33--39

Morphological, physico-chemical and structural characterization of mucilage isolated from the seeds of Buchanania lanzan Spreng


Sudarshan Singh1, Sunil B Bothara2 
1 Department of Pharmaceutics, H.S.B.P.V.T.'s Group of Institutions, College of Pharmacy, Kashti, Ahmednagar, Maharashtra, India
2 Department of Pharmacology, Shree G. M. Bilakhia College of Pharmacy Rofel, Vapi, Gujarat, India

Correspondence Address:
Sudarshan Singh
H.S.B.P.V.TSQs Group of Institutions, College of Pharmacy, Kashti, Ahmednagar 414 701, Maharashtra
India

Context: Mucilage isolated from the seeds of Buchanania lanzan a wild Indian plant. This mucilage can be commercially exploited, by evaluating physico-chemical properties of this mucilage. Materials and Methods: Various physico-chemical parameter using scanning electron microscopy (SEM), molecular weight, X-ray diffraction (XRD) spectrometry, zeta potential (ZP), Fourier transform infrared (FT-IR) spectroscopy and 1D ( 1 H and 13 C) nuclear magnetic resonance (NMR) have been employed to characterize mucilage in the present study. Results: SEM analysis suggests that the mucilage has irregular particle size. Thermogravimetry analysis suggested that mucilage had good thermal stability with two stage decomposition. The weight-average molecular weight of mucilage was determined to be 4883, by gel permeation chromatography. The XRD pattern of the mucilage indicated a completely amorphous structure. The ZP was obtained 1.56 and −9.49 mV in water and 0.1 N NaCl respectively. The major functional groups identified from FT-IR spectrum include 3459/cm (-OH), 1667/cm (Alkenyl C-H and C = C stretch), 1407/cm (-COO-) and 1321/cm (-CH 3 CO). Analysis of mucilage by paper chromatography and 1D NMR indicated the presence of rhamnose, arabinose and fructose. Conclusion: The spectral and chromatography analysis of mucilage indicate that the mucilage is composed of basic sugar moiety such as (arabinose, rhamnose, fructose and mannose) as complex carbohydrates.


How to cite this article:
Singh S, Bothara SB. Morphological, physico-chemical and structural characterization of mucilage isolated from the seeds of Buchanania lanzan Spreng.Int J Health Allied Sci 2014;3:33-39


How to cite this URL:
Singh S, Bothara SB. Morphological, physico-chemical and structural characterization of mucilage isolated from the seeds of Buchanania lanzan Spreng. Int J Health Allied Sci [serial online] 2014 [cited 2020 Apr 7 ];3:33-39
Available from: http://www.ijhas.in/article.asp?issn=2278-344X;year=2014;volume=3;issue=1;spage=33;epage=39;aulast=Singh;type=0