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Year : 2016  |  Volume : 5  |  Issue : 2  |  Page : 88-92

Evaluation of two diagnostic methods for dengue virus infection and its correlation with thrombocytopenia

Department of Microbiology, Government Medical College, Surat, Gujarat, India

Correspondence Address:
Tanvi H Panwala
Department of Microbiology, Government Medical College, Surat - 395 001, Gujarat
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2278-344X.180426

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Context: Early definitive diagnosis of dengue virus (DENV) infections is essential for the timely management of dengue infections. Aims: The aim of the present study is to compare results of both tests (IgM enzyme-linked immunosorbent assay [ELISA] and nonstructural protein 1 [NS1]) and to prove that use of NS1 with IgM ELISA tests improves the dengue laboratory diagnosis. Settings and Design: Tertiary Care Hospital, Gujarat and retrospective cross-sectional study. Subjects and Methods: One thousand three hundred forty-three blood samples were screened from clinically suspected cases of dengue fever reporting at Tertiary Care Hospital, Gujarat from August to November 2014. The samples were divided into three study groups according to the tests performed. In Group 1, 840 samples were subjected for dengue IgM ELISA only. Group 2 comprised 379 samples tested for NS1 antigen (Ag) only. Group 3 comprised 124 samples tested by both methods NS1 Ag detection test and IgM ELISA. Statistical Analysis Used: As the study has compared three different groups, Statistical proportion method used and data entry was done in Microsoft office excel version 2010. Results: In Group 1, 145 patients (76.3%) found positive between 6 and 10 days of fever. More number of cases (89.4%) gave positive reaction in NS1 Ag detection test at <5 days of fever in Group 2. In Group 3, of the forty positive samples, 19 (47%) samples gave positive reaction in NS1 Ag detection test and negative in IgM ELISA with <5 days of fever history. Ten patients (25%) showed positive IgM ELISA and negative for NS1 at more than 10 days of fever. Eleven patients (27.5%) showed both the tests positive between 6 and 10 days of fever. Conclusions: If NS1 Ag detection test used in combination with IgM ELISA on a single serum sample of suspected patients will increase the sensitivity of detection, especially in areas with higher prevalence of secondary DENV infections.

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